Journal: Cell Reports Medicine

Article Title: Preclinical development of a chimeric antigen receptor T cell therapy targeting FGFR4 in rhabdomyosarcoma

doi: 10.1016/j.xcrm.2023.101212

Figure Lengend Snippet: Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal epithelial cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).

Article Snippet: Human cardiomyocytes (Cat# 36044-15), cholangiocytes (Cat# 36755-12), and pancreatic epithelial primary cells (Cat# 36099-25) were obtained from Cellprogen; human coronary artery smooth muscle cells (CASMC, Cat# CC-2583), small airway epithelial cells (SAEC, Cat# CC-2547), bronchial/tracheal epithelial cells (NHBE, Cat# CC-2541), human renal epithelial cells (HRE, Cat# CC-2556), renal cortical epithelial cells (HREC, Cat# CC-2554), renal proximal tubule epithelial cells (RPTEC, Cat# CC-2553), hepatocytes (Cat# HUCPG), and 10 Donors pooled hepatocytes (Cat# HUCS10P) were purchased from Lonza; HEK 293 cells (Cat# CRL-1573) was purchased from ATCC.

Techniques: Flow Cytometry, Expressing, Control, Staining

Journal: Cell Reports Medicine

Article Title: Preclinical development of a chimeric antigen receptor T cell therapy targeting FGFR4 in rhabdomyosarcoma

doi: 10.1016/j.xcrm.2023.101212

Figure Lengend Snippet:

Article Snippet: Human cardiomyocytes (Cat# 36044-15), cholangiocytes (Cat# 36755-12), and pancreatic epithelial primary cells (Cat# 36099-25) were obtained from Cellprogen; human coronary artery smooth muscle cells (CASMC, Cat# CC-2583), small airway epithelial cells (SAEC, Cat# CC-2547), bronchial/tracheal epithelial cells (NHBE, Cat# CC-2541), human renal epithelial cells (HRE, Cat# CC-2556), renal cortical epithelial cells (HREC, Cat# CC-2554), renal proximal tubule epithelial cells (RPTEC, Cat# CC-2553), hepatocytes (Cat# HUCPG), and 10 Donors pooled hepatocytes (Cat# HUCS10P) were purchased from Lonza; HEK 293 cells (Cat# CRL-1573) was purchased from ATCC.

Techniques: Purification, Control, Recombinant, Labeling, Virus, Microarray, Cytokine Assay, Transfection, Chromatin Immunoprecipitation, ChIP-sequencing, Plasmid Preparation, Software, Imaging